Production and Characterization of Amelogenin-ELP Fusion Proteins
This study aimed to engineer and characterize a series of Amelogenin-ELP fusion proteins to explore their potential as biomaterials with enhanced properties. Eleven constructs (176-186) were designed and expressed in E. coli BL21(DE3) cells, with successful purification confirmed by SDS-PAGE. Dynamic Light Scattering (DLS) revealed that constructs 181, 185, and 186 formed nanospheres similar to fu