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Mycobacterial ESX-1 may target mitochondria to modulate the immune response

Tuberculosis (TB) is an infectious disease caused by a bacterium called Mycobacterium tuberculosis (Mtb). Infection occurs through inhalation of bacteria-containing droplets. After entry into the respiratory tract the bacteria mainly reside within macrophages in the lung by using different strategies to escape killing by the immune system. The attenuated Mycobacterium bovis strain Bacille Calmette

CONSUMER PERCEPTIONS OF THE FAST FOOD INDUSTRY IN SWEDEN.

The fast food industry is one of the important and growing segments of the overall restaurant industry. At the same time, it is a very dynamic and competitive business where too many vendors are chasing too few clients.To maintain sustainable growth and long-term success it is crucial to meet consumer needs and offer value to them. In order to achieve this, fast food providers must first evaluate

Legal Obedience in Small Business Owners ­- observations of attitudes and behaviours towards rules and regulations

This research study aims to examine why small business owners comply with rules and regulations within their industries. The purpose is to obtain a deeper understanding of legal obedience amongst small business owners. More specifically, what extrinsic and intrinsic motivations makes small business owners comply with the laws. This study is exploratory and is based on five semi-­structured intervi

Properties of fractionated and blended Polyhydroxyalkanoates

The purpose of this project was to gain knowledge of polyhydroxyalkanoates’ (PHAs’) ability to be fractionated and form miscible blends for different structures, by using the methods: solvent/non solvent fractionation and co-dissolving in 2-butanol.The outcome of the fractions and blends was evaluated with differential scanning calorimetry (DSC), size exclusion chromatography (SEC), Fourier transf

Extern varvtalsmätning på en permanentmagnetiserad likströmsmotor

This bachelor thesis was performed at BorgWarner AB in Landskrona. The assignment was to investigate different methods for external measurement of a permanent magnetized DC motor speed in revolutions per minute when you have no access to the rotor. The task was to examine different measurement methods, to produce a finished circuit that converts the signal frequency to rpm and build a complete too

Using Cooperative Game Theory to Analyse Allocations of Costs Related to Connecting Renewable Energy to the Power Grid

Building new wind farms is an important part in the transition to renewable energy. However these projects often require large investments, and the cost of upgrading the existing infrastructure - to allow for the connection of new parks - is a component that cannot be ignored. When several wind farms seek interconnections, they are forced to collaborate and financing the connection. This leads to

Immune function varies between short- and long-distance migrants during autumn migration

Do sick birds migrate at different times than healthy ones? The word migration comes from the Latin migratus that means “to change”, referring to how birds change their geographic locations seasonally. Birds migrate to escape from decreased food availability or harsh weather on their breeding grounds. They migrate to areas of high or increasing resources where they stay during the winter period b

Engineering new molecular tools to study orphan small nucleolar RNAs in human cells

Recent advances in high-throughput sequencing have uncovered that the vast majority of the cellular genome encodes for a myriad of noncoding RNAs, which lack coding potential for proteins. Increasing evidence suggests that ncRNA classes are involved in important processes that help in decoding the genetic information within cells. A striking example is the family of small nucleolar RNAs (snoRNAs)

Antibody Binding Epitopes of an NTHi Moonlighting Protein

After the introduction of a conjugate vaccine that provides immunization to Haemophilus influenzae type b, an increase in the number of infections caused by Non-typeable H. influenzae (NTHi) has been reported. NTHi mostly affects children under the age of 1, causing cases of otitis media and conjunctivitis, and it is also associated with respiratory tract infections such as exacerbations of chroni

Helicobacter pylori catalase (KatA) and KatA-associated protein (KapA) interaction

Catalase transport in Helicobacter pylori Helicobacter pylori is a bacterium that lives in the human stomach of 50% of the world’s population. Its presence in the stomach induces a strong immune response in all these cases and the body’s response to fight off the infection causes an irritation of the stomach that may be symptomatic or asymptomatic. A persistent infection may lead to ulcers or in

CRISPR-Cas9-Mediated DGCR8 Deletion in a Human Stem Cell Line and Exploration of In Vivo Applications

The CRISPR-Cas9 system for genetic engineering has recently emerged as promising tool for studying gene functions in a variety of systems and organisms. Here, we are particularly interested in studying the role of DGCR8 in a human neuroepithelial-like stem (hNES) cell line. Beyond its characterized role in the microRNA biogenesis pathway, recent studies have implicated the enzyme’s involvement in

Experimental evolution in biofilm models of Pseudomonas aeruginosa using mutagenesis screening reporters

What does not kill bacteria, makes them stronger Bacteria most often gather together to form communities called biofilms, which represent the underlying cause of 60% of all infections. Bacteria hidden in the biofilms cannot be eliminated by the human immune system and by antibiotics, therefore, they cause persistent infections which require long-term antibiotic therapy. One of the unwanted effect

Genomic and metabolic response of citrate in human lung fibroblasts

Citrat ger cellerna mer energi Njurarna är ett organ i kroppen vars huvuduppgift är att avlägsna överflödig vätska och rena blodet från restprodukter. Njursvikt innebär ett tillstånd där njurarna helt eller till största delen förlorat sin funktion och kan leda till ett livshotande tillstånd då vätskenivån i kroppen ökar. Detta kan bl.a. leda till ett förhöjt blodtryck och att nivån av olika giftiObjective: Citrate is widely used in dialysis, either as an acid in dialysis fluids or as a regional anticoagulant. In both cases, the concentration of citrate in the plasma of the patients will increase above physiological levels, up to 5-foldes in some cases. The aim of this study was to investigate the effect of citrate on the transcriptional level, as well as on the metabolic level and elucida

Artificial Intelligence and Terrain Handling of a Hexapod Robot

The focus of this master’s thesis has been getting a six-legged robot to autonomously navigate around a room with obstacles. To make this possible a time-of-flight camera has been implemented in order to map the environment. Two force sensing resistors were also mounted on the front legs to sense if the legs are in contact with the ground. The information from the sensors together with a path plan

Design of a Marine Protected Area in Öresund (Baltic Sea) and its effects on population of Atlantic cod (Gadus morhua)

Marine protected areas (MPA) have been widely used the recent years, as an alternative to the traditional management tools, in order to increase the yield and the health of a stock. The present study shows how cod population in Öresund would respond under different spatial and temporal MPA and how they will affect the gill-net fisheries in the area. For that reason, a simple model, based on logist

I nationens intresse? EU-rättens reglering av och kommissionens syn på statsstöd till flygsektorn och statliga flygbolag

Den europeiska flygmarknaden har under nästan hela 1900-talet dominerats av nationella flygbolag som har subventionerats för att kunna upprätthålla stora linjenät och en hög servicenivå. Flygbolagen var länge undantagna från Europeiska unionens konkurrens- och statsstödsregler men kraven på en avreglering ökade under 1980-talet. Efter en liberaliseringsprocess mellan 1987 och 1993 öppnades marknadUp until the 1990’s, the European aviation market was dominated by state owned national airlines that were subsidized by their respective states in order to maintain large networks and a high level of service. The airline industry was excluded from EU regulations of competition and state aid. However, higher demands for a deregulation of the market were raised in the 1980’s. After a liberalization

Letters of intent – ur praktisk synvinkel

I samband med kommersiella avtalsförhandlingar upprättas många olika förhandlingsdokument. Parterna kan skriva sekretessavtal, letters of intent, aktieägaravtal, anställningsavtal och överlåtelseavtal – för att nämna några. Under förhandlingarnas gång skickas många förhandlingsbud och åtskilliga avtalsförslag, avseende det slutliga överlåtelseavtalet, mellan parterna. Ett letter of intent bringaIn a contract negotiation a variety of negotiation documents are created. The parties may sign confidentiality agreements, letters of intent, shareholder agreements, employment agreements and transfer agreements – to name a few. During negotiations, many offers and proposals regarding the final transfer agreement are exchanged between the parties. A letter of intent brings order and clarity in a

Från livstid till viss tid - om tidsbestämning av livstidsstraff

När lag (2006:45) om omvandling av fängelse på livstid trädde ikraft år 2006 innebar det att ett nytt system för frigivning av livstidsdömda infördes i svensk rätt. Systemet var tänkt att gälla som ett komplement till nådeinstitutet som under lång tid varit en livstidsdömds enda möjlighet att bli frigiven från sitt straff. Avsikten med införandet av lagen var att den skulle medföra en förbättring In 2006, a new system for release of prisoners serving a life sentence was introduced in Sweden by the law (2006:45) on conversion of imprisonment for life (”omvandlingslagen”). It was meant to serve as a complement to the government’s right to pardon, which for a long time has been the only possibility for a prisoner to be released from his or her life sentence. The law on conversion of imprisonm

A Diligent Businessman's Guide to Defence of State Aid Recovery - Why it will not succeed and why not to worry

Återkrav av felaktigt utbetalt statsstöd är en grundbult i statstödssystemet. Statsstöd som är oförenligt med den inre marknaden, artikel 107 FEUF, och som strider mot genomförandeförbudet i artikel 108(3, ska återkrävas i sin helhet. Stöd som enbart strider mot genomförandeförbudet medför krav på att mottagaren ska betala ränta. Kravet på återbetalning får dock inte strida mot en unionsprincip, Recovery of state aid is a pillar of the state aid control system. Aid in breach of Article 107 and 108(3) TFEU, incompatible aid, is retrieved in full, whereas aid which is only in breach of the standstill obligation, Article 108(3), is the subject to payment of interest. The Union acknowledges three exemptions to the recovery obligation: a recovery is in breach of Union principles, the aid was p

Stödbevisning i våldtäktsmål - en problematisk rättstillämpning?

Enligt grundläggande principer följer att det råder fri bevisföring och fri bevisvärdering. Därmed kan det i princip inte uppställas några legala regler för vilka bevis som får åberopas eller vilka metoder som ska eller bör tillämpas av domstolen vid bevisvärderingen. Därmed är det teoretiskt möjligt att enbart en målsägandeutsaga kan till ligga till grund för att beviskravet ska anses uppnått ochAccording to the fundamental principle of free assessment of evidence, there can be no legal constraints to which evidence that are allowed or by which methods such evidence shall be assessed by the courts. Thus, it is theoretically possible to pass sentence in a criminal case concerning rape only on the basis of the injured party´s testimony. Even so, case law indicates that it is rarely enough w